Gene name |
SPCC550.11 |
Gene ID |
48/C05 |
Gene synonyms/obsolete |
|
Gene product |
RanBP7/importin-beta/Cse1p family; RanGTP-binding
protein; involved in mRNA export; involved in mRNA decay |
Entry clone |
Cloned |
ORF length (unspliced) |
3427 |
ORF length (spliced) |
3090 |
Entry clone length |
3427 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC550.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTGGTTGAGCATTT |
Rev primer name |
SPCC550.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTTTATCGGAAGCGACA |
Amino acid length |
1029 |
Molecular weight |
116.4 |
Isoelectric point (calc.) |
4.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPVRIQAALAL |
Localization (YFP) |
nuclear envelope;
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |