Gene name |
SPAC13G6.01c |
Gene ID |
48/C04 |
Gene synonyms/obsolete |
rad8;
SPAC5H10.14c |
Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); SNF2
family; DEAD/DEAH box helicase; involved in DNA repair;
helicase C-terminal domain; involved in nucleotide-excision
repair |
Entry clone |
Cloned |
ORF length (unspliced) |
3402 |
ORF length (spliced) |
|
Entry clone length |
3402 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC13G6.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAAGAAAGGTACAAAA |
Rev primer name |
SPAC13G6.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATATTCAAACAACATTTTG |
Amino acid length |
1133 |
Molecular weight |
128.6 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
nuclear dots by over
expression? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |