| Gene name |
SPAC824.02 |
| Gene ID |
48/C06 |
| Gene synonyms/obsolete |
|
| Gene product |
negative regulator of
vesicle formation; negative regulator of COPII vesicle
formation; involved in intracellular protein transport |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3429 |
| ORF length (spliced) |
|
| Entry clone length |
3429 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC824.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGACGATAAGGGAAG |
| Rev primer name |
SPAC824.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAAATAGATCGCAAAAAT |
| Amino acid length |
1142 |
| Molecular weight |
129.4 |
| Isoelectric point (calc.) |
9.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYVAIIAILLL/LLFLVSILSI/LLTLMFVGLVI/LYFFWLLFL |
| Localization (YFP) |
ER; Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |