Gene name |
SPAC22H10.03c |
Gene ID |
48/B05 |
Gene synonyms/obsolete |
kap114 |
Gene product |
ranbp7-importin-beta-cse1p family protein |
Entry clone |
Cloned |
ORF length (unspliced) |
3242 |
ORF length (spliced) |
2961 |
Entry clone length |
3242 |
No. of intron |
6 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC22H10.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTGAAAGCAAAATCAT |
Rev primer name |
SPAC22H10.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTCTTTAATTTGAACG |
Amino acid length |
986 |
Molecular weight |
111 |
Isoelectric point (calc.) |
4.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSCIKRLAI |
Localization (YFP) |
faint nuclear
envelope; nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |