Gene name |
SPCC4E9.01c |
Gene ID |
48/B04 |
Gene synonyms/obsolete |
rec11;
SPCC550.16c |
Gene product |
region specific
activator of recombination; involved in meiotic recombination
(required); involved in sister chromatid cohesion; cohesin
complex (meiotic); chromosome arm cohesin complex (meiotic);
low similarity to WD repeat protein (probably spurious);
similar to Sp rec7 (paralog); no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
3240 |
ORF length (spliced) |
2772 |
Entry clone length |
3240 |
No. of intron |
8 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC4E9.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGATTCGAATTTGAATC |
Rev primer name |
SPCC4E9.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGATAAATGGTCAGCTGTT |
Amino acid length |
923 |
Molecular weight |
107.4 |
Isoelectric point (calc.) |
5.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFKMNALLFI/LKVLFLLLL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |