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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G6.16c
Gene ID 47/A07
Gene synonyms/obsolete
Gene product involved in polyadenylation
Entry clone Cloned
ORF length (unspliced) 2638
ORF length (spliced) 2328
Entry clone length 2638
No. of intron 5
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC17G6.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCAAAAGAAAGGAATT
Rev primer name SPAC17G6.16.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAAAGTTCTTCTTTCTTA
Amino acid length 775
Molecular weight 88
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSTVDVLLI
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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