| Gene name |
SPAP8A3.09c |
| Gene ID |
40/H01 |
| Gene synonyms/obsolete |
paa1 |
| Gene product |
protein phosphotase 2A
(regulatory subunit); involved in cellular morphogenesis;
involved in cell wall biosynthesis; involved in
cytokinesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2147 |
| ORF length (spliced) |
1773 |
| Entry clone length |
2147 |
| No. of intron |
6 |
| Sequence status |
Finished |
| Sequence results |
1541T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAP8A3.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAACAGAGAATGTGTG |
| Rev primer name |
SPAP8A3.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCATTTGTTTGCTCTAAG |
| Amino acid length |
590 |
| Molecular weight |
66.6 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |