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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13C5.07
Gene ID 40/H02
Gene synonyms/obsolete rad32
Gene product exonuclease; involved in DNA repair; involved in double-strand break repair (required); involved in meiotic recombination (required); involved in meiotic gene conversion (required); involved in end joining; involved in telomere maintenance; involved in telomerase-dependent telomere maintenance; involved in DNA damage sensitivity; involved in DNA replication; Mre11 complex (multisubunit endonuclease); phosphoprotein; essential phosphoesterase motif
Entry clone Cloned in 2006 trial
ORF length (unspliced) 2156
ORF length (spliced) 1950
Entry clone length 2156
No. of intron 4
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC13C5.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCAAATGACCCCTCAGA
Rev primer name SPAC13C5.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATCATCTAAAATTTCGTCA
Amino acid length 649
Molecular weight 73.6
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 574
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYQALRSLRL/LQKGFTKLAL
Localization (YFP) no expression clone
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation

Image information
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