| Gene name |
SPAPYUG7.02c |
| Gene ID |
40/G12 |
| Gene synonyms/obsolete |
sin1 |
| Gene product |
stress activated map
kinase interacting protein; interacts physically with Sty1p;
eukaryotic SAPK pathway |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2096 |
| ORF length (spliced) |
1998 |
| Entry clone length |
2096 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1319T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAPYUG7.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATTAACAAGAGAGAA |
| Rev primer name |
SPAPYUG7.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTATTTTTTTAACAGTA |
| Amino acid length |
665 |
| Molecular weight |
74 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDKSLQDLLI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |