Gene name |
SPBC17G9.09 |
Gene ID |
40/D10 |
Gene synonyms/obsolete |
tif213 |
Gene product |
translation initiation
factor 2 (gamma subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
1341 |
ORF length (spliced) |
|
Entry clone length |
1341 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC17G9.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGGAAAATCTTGATAT |
Rev primer name |
SPBC17G9.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACTTTTAGAGTCTTACCC |
Amino acid length |
446 |
Molecular weight |
48.7 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSGNVDPLPI/LKEAVLCLAL |
Localization (YFP) |
cytosol; cytoplasmic
dots |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus; cytoplasmic dots |
Microscope used for
observation |
DeltaVision |