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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19A8.02
Gene ID 39/C06
Gene synonyms/obsolete
Gene product hypothetical protein involved in transcriptional activation; pleckstrin homology domain; possibly protein kinase interacting
Entry clone Cloned
ORF length (unspliced) 3740
ORF length (spliced) 3642
Entry clone length 3740
No. of intron 1
Sequence status Finished
Sequence results 960T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19A8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCGCTCCTGCAAATGC
Rev primer name SPAC19A8.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGCAGCGTAGTCTGCGTTT
Amino acid length 1213
Molecular weight 140.5
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRKFIVAPLDL
Localization (YFP) periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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