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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC922.08c
Gene ID 35/H10
Gene synonyms/obsolete SPAC869.11
Gene product APC amino acid transporter (putative); similar to Sp SPBC359.01C and SPBC359.03C and SPAP7G5.06 and SPBPB2B2.01 and ISP5(paralog); closely related paralogous family; amino acid permease family; similar to Sp SPAP7G5.06 and SPBC359.01 and meu22 and SPBPB2B2.01 and isp5
Entry clone Cloned
ORF length (unspliced) 1743
ORF length (spliced)
Entry clone length 1743
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC922.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACCAGAATATGTCTC
Rev primer name SPAC922.08.Rv
Rev primer SEQ AGAAAGCTGGGTAACAGAAAACCGAACTGATT
Amino acid length 580
Molecular weight 63.6
Isoelectric point (calc.) 7.9
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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