| Gene name |
SPAC1002.11 |
| Gene ID |
35/H11 |
| Gene synonyms/obsolete |
|
| Gene product |
GPI-anchor
transamidase complex; involved in GPI anchor biosynthesis;
involved in attachment of GPI anchor to protein |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1746 |
| ORF length (spliced) |
|
| Entry clone length |
1746 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
29G:T |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1002.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTCTATTCACTTTTGT |
| Rev primer name |
SPAC1002.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAAGTCTTTGTCTTGGTT |
| Amino acid length |
581 |
| Molecular weight |
66.8 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQRHLFFLQL/LMPSVFMILVL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |