| Gene name |
SPAC19D5.04 |
| Gene ID |
34/D06 |
| Gene synonyms/obsolete |
|
| Gene product |
HECT domain;
ubiquitin-protein ligase (E3) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
9684 |
| ORF length (spliced) |
|
| Entry clone length |
9684 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1144A:G / 1603C:T /
1619T:C / 1689T:C / 1705C:T / 1809T:C / 5281A:G / 8953T:C /
9541A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC19D5.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGGATAACGAAAAGTCC |
| Rev primer name |
SPAC19D5.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCAAATCCAAAACCTTCA |
| Amino acid length |
3227 |
| Molecular weight |
365 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEVIDSTLAL/LMIRIGLLAL/LKNLLKFVLHL/LSSTLLNLIL/LGPAVGLLSL/LSELNFFLEL/LERFKTLFI |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |