Gene name |
SPAC29B12.07 |
Gene ID |
34/C01 |
Gene synonyms/obsolete |
sec16 |
Gene product |
multidomain vesicle
coat component; involved in ER to Golgi transport; involved in
vesicle formation |
Entry clone |
Cloned# |
ORF length (unspliced) |
5988 |
ORF length (spliced) |
|
Entry clone length |
5988 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC29B12.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATATTGAATCAGACCA |
Rev primer name |
SPAC29B12.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACTATTTCTCATGACATCC |
Amino acid length |
1995 |
Molecular weight |
218 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSGVGVNLQL/LRVLGTLLL |
Localization (YFP) |
cytoplasmic dots,
especially at site of septum formation |
Comments for localization |
a lot of dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |