| Gene name |
SPAC29B12.07 |
| Gene ID |
34/C01 |
| Gene synonyms/obsolete |
sec16 |
| Gene product |
multidomain vesicle
coat component; involved in ER to Golgi transport; involved in
vesicle formation |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
5988 |
| ORF length (spliced) |
|
| Entry clone length |
5988 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC29B12.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATATTGAATCAGACCA |
| Rev primer name |
SPAC29B12.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTATTTCTCATGACATCC |
| Amino acid length |
1995 |
| Molecular weight |
218 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSGVGVNLQL/LRVLGTLLL |
| Localization (YFP) |
cytoplasmic dots,
especially at site of septum formation |
| Comments for localization |
a lot of dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |