Gene name |
SPBP8B7.32c |
Gene ID |
32/D10 |
Gene synonyms/obsolete |
puc1;
SPBC19F5.01c |
Gene product |
G1-type cyclin;
involved in exit from the mitotic cycle |
Entry clone |
Cloned |
ORF length (unspliced) |
1080 |
ORF length (spliced) |
|
Entry clone length |
1080 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP8B7.32.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTAGTTTCTTCCAATGA |
Rev primer name |
SPBP8B7.32.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAAGTACGCTCAGTATCC |
Amino acid length |
359 |
Molecular weight |
40.4 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDPIFFLVL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |