| Gene name |
SPAC6F6.01 |
| Gene ID |
31/B08 |
| Gene synonyms/obsolete |
|
| Gene product |
VIC sodium
channel |
| Entry clone |
Cloned |
| ORF length (unspliced) |
5565 |
| ORF length (spliced) |
|
| Entry clone length |
5565 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
728A:G / 2119A:G /
4650T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6F6.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATCAAGCTCAAACTC |
| Rev primer name |
SPAC6F6.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCCTTTAAATGAATCCCGA |
| Amino acid length |
1854 |
| Molecular weight |
213.1 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
23 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYTLEMLLKI/LFSMIACLRL/LTLWLMSLVI/LVDIVLAVLNL/LFSNFKQLLNL/LFLVIVLFI/LVSMIYILTL/LYIVHLLFL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |