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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC5E4.04
Gene ID 31/B09
Gene synonyms/obsolete cut1
Gene product separin; separase; involved in mitotic regulation and sister chromatid separation; promotes anaphase; loaded onto the metaphase spindle by Cut2p; peptidase family C50; shows proteolytic activity toward Rad21/Scc1
Entry clone Cloned
ORF length (unspliced) 5605
ORF length (spliced) 5487
Entry clone length 5605
No. of intron 3
Sequence status Finished
Sequence results 1573T:C / 5236A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC5E4.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTACAAGGTCAATCGT
Rev primer name SPCC5E4.04.Rv
Rev primer SEQ AGAAAGCTGGGTATGGAATAATATAAGCAGGT
Amino acid length 1828
Molecular weight 209.4
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 1717
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LNVLRCLSL/LQSFKLLIL/LHTTFNQLDL/LTFELAFLEI/LHLQFSKYLVI/LSRTVSLNLLL/LTKGIKRLSL/LDYLRERLKI/LEQILNSRLSI/LSPEILELFI/LEDLIYFILDI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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