| Gene name |
SPCC290.03c |
| Gene ID |
31/A09 |
| Gene synonyms/obsolete |
|
| Gene product |
nucleoporin |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4995 |
| ORF length (spliced) |
4944 |
| Entry clone length |
4995 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
105A:G / 374T:C /
831T:C / 2822T:C / 4456A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC290.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGTCTGGGAATCTCA |
| Rev primer name |
SPCC290.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTCTATAAAGATCCAAG |
| Amino acid length |
1647 |
| Molecular weight |
186.4 |
| Isoelectric point (calc.) |
5.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSLTLSTQLNL/LAVLDGLNI/LCILRNVLSL/LEFMDILRI/LKPPVQRLMI/LLQIFILVL |
| Localization (YFP) |
SPB?; nuclear dots;
nucleus>cytosol; nuclear envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |