| Gene name |
SPBC23E6.04c |
| Gene ID |
31/A08 |
| Gene synonyms/obsolete |
|
| Gene product |
ubiquitin-protein
ligase (E3); associates with the 26S proteasome; HECT domain;
ankyrin or armadillo repeats |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4950 |
| ORF length (spliced) |
|
| Entry clone length |
4950 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
2846A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC23E6.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTCTTCACTTCAGAA |
| Rev primer name |
SPBC23E6.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGTAAGGTAATCTTGGAGG |
| Amino acid length |
1649 |
| Molecular weight |
186.4 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LECVQILRL/LLSSIAASLPL/LNLILTFLEL/LSNVRVLLL/LADIVNYSLSI/LLELIELAL |
| Localization (YFP) |
large SPB |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |