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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC12B10.01c
Gene ID 31/A07
Gene synonyms/obsolete SPAC31F12.02c; SPAC637.15c
Gene product DEAD/DEAH box helicase
Entry clone Cloned
ORF length (unspliced) 4944
ORF length (spliced)
Entry clone length 4944
No. of intron 0
Sequence status Finished
Sequence results 356T:C / 558A:G / 2380G:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC12B10.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGTTCAAGCGTATAC
Rev primer name SPAC12B10.01.Rv
Rev primer SEQ AGAAAGCTGGGTAGGATAAATGAAAACTGCCC
Amino acid length 1647
Molecular weight 185.6
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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