| Gene name |
SPCC320.08 |
| Gene ID |
24/F05 |
| Gene synonyms/obsolete |
|
| Gene product |
DUF6; no apparent Sc
ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1518 |
| ORF length (spliced) |
|
| Entry clone length |
1518 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1111A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC320.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTGACGGTTCGATACA |
| Rev primer name |
SPCC320.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACGACTTTCTGACAAAGACA |
| Amino acid length |
505 |
| Molecular weight |
56.7 |
| Isoelectric point (calc.) |
5.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
10 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGQVLSLCI/LEEYFASKLPL/LTSDFWSLVI/LYPIAFVLII |
| Localization (YFP) |
Golgi; periphery,
especially at cell tip and site of septum formation; vacuole
membrane |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |