Gene name |
SPAC56F8.05c |
Gene ID |
23/G07 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein; conserved yeast protein; similar to S.
cerevisiae YIL041W |
Entry clone |
Cloned |
ORF length (unspliced) |
1474 |
ORF length (spliced) |
888 |
Entry clone length |
1474 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC56F8.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATTCGACATTGGTAAA |
Rev primer name |
SPAC56F8.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGATGGTTATATCCGATAGA |
Amino acid length |
295 |
Molecular weight |
32.7 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
cytoplasmic dots,
especially near site of septum formation |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |