| Gene name |
SPAC2G11.10c |
| Gene ID |
23/G06 |
| Gene synonyms/obsolete |
|
| Gene product |
molybdopterin synthase
sulfurylase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1474 |
| ORF length (spliced) |
1206 |
| Entry clone length |
1474 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
991T:C / 1115A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC2G11.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCCACAAGAGAAAAT |
| Rev primer name |
SPAC2G11.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATACAGTGGGAAATTTGGG |
| Amino acid length |
401 |
| Molecular weight |
44.4 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LETVKLILHI |
| Localization (YFP) |
nuclear dots;
cytoplasmic dots; nucleus>cytosol |
| Comments for localization |
moving, small nuclear
dots; cytoplasmic aggregates |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |