Gene name |
SPBC3H7.06c |
Gene ID |
23/G08 |
Gene synonyms/obsolete |
pof9 |
Gene product |
F-box protein |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1474 |
ORF length (spliced) |
1404 |
Entry clone length |
1474 |
No. of intron |
1 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC3H7.06c.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTAAATCACCATTCCT |
Rev primer name |
SPBC3H7.06c.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCCGTCCCTTTCCAAAGTC |
Amino acid length |
467 |
Molecular weight |
52.4 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |