| Gene name |
SPBC651.07 |
| Gene ID |
15/C09 |
| Gene synonyms/obsolete |
|
| Gene product |
COP9/signalosome
associated (pers. comm. Tony Carr); no apparent ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1022 |
| ORF length (spliced) |
807 |
| Entry clone length |
1022 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
61A:T / 497T:C /
732T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC651.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGTAATGTACGTTCC |
| Rev primer name |
SPBC651.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAACCATTACTATAGACT |
| Amino acid length |
268 |
| Molecular weight |
30.1 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVGPIVQTLSI/LVTSVREILEL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |