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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1D4.13
Gene ID 15/C10
Gene synonyms/obsolete ste1; byr1; ste3
Gene product serine/threonine protein kinase; MAP kinase kinase (MAPKK); involved in conjugation; involved in sporulation; pheromone signalling pathway
Entry clone Cloned#
ORF length (unspliced) 1023
ORF length (spliced)
Entry clone length 1023
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1D4.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTAAACGACGTCGAAA
Rev primer name SPAC1D4.13.Rv
Rev primer SEQ AGAAAGCTGGGTACGAGGATCTAAAGTTGGAG
Amino acid length 340
Molecular weight 38.1
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LIYLYNVLHI/LPSSFPEDLRL
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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