Gene name |
SPBP35G2.03c |
Gene ID |
15/C08 |
Gene synonyms/obsolete |
|
Gene product |
sequence orphan;
hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1022 |
ORF length (spliced) |
960 |
Entry clone length |
1022 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
291A:G / 454A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBP35G2.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACTTTCAATTTATAAA |
Rev primer name |
SPBP35G2.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCTTTGCCTCTGGGATGT |
Amino acid length |
319 |
Molecular weight |
37.1 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nuclear dots; nuclear
envelope? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |