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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP35G2.03c
Gene ID 15/C08
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1022
ORF length (spliced) 960
Entry clone length 1022
No. of intron 1
Sequence status Finished
Sequence results 291A:G / 454A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP35G2.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACTTTCAATTTATAAA
Rev primer name SPBP35G2.03.Rv
Rev primer SEQ AGAAAGCTGGGTATGCTTTGCCTCTGGGATGT
Amino acid length 319
Molecular weight 37.1
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nuclear envelope?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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