| Gene name |
SPCC1795.04c |
| Gene ID |
12/G02 |
| Gene synonyms/obsolete |
|
| Gene product |
20S proteasome
component (alpha 7) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
916 |
| ORF length (spliced) |
762 |
| Entry clone length |
916 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1795.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGTTAGCAAGGAGT |
| Rev primer name |
SPCC1795.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCTTGCATAGCAATATCC |
| Amino acid length |
253 |
| Molecular weight |
28 |
| Isoelectric point (calc.) |
4.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
nuclear
envelope? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |