Gene name |
SPAC637.10c |
Gene ID |
12/G01 |
Gene synonyms/obsolete |
rpn10; pus1 |
Gene product |
19S proteasome
regulatory subunit; ubiquitin-binding protein; UBA domain; VWA
domain; involved in protein deubiquitination |
Entry clone |
Cloned |
ORF length (unspliced) |
916 |
ORF length (spliced) |
732 |
Entry clone length |
916 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC637.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGTTAGAAGCAACGAT |
Rev primer name |
SPAC637.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCTTGCATTTTTTTATCC |
Amino acid length |
243 |
Molecular weight |
27.1 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDVELALALEL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
nuclear
envelope? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |