Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC31F10.07
Gene ID 12/F12
Gene synonyms/obsolete
Gene product actin cortical patch component (putative); involved in actin cytoskeletal organization; involved in endocytosis; couples endocytosis to the cytoskeleton
Entry clone Cloned
ORF length (unspliced) 915
ORF length (spliced)
Entry clone length 915
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC31F10.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAATTTTCAGTGAAAC
Rev primer name SPBC31F10.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTCCAAACGATTATGATCG
Amino acid length 304
Molecular weight 34.1
Isoelectric point (calc.) 7.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) SPB; cytoplasmic dots at periphery, especially at cell tip and site of septum formation; nucleus>cytosol
Comments for localization asymmetrical localization of SPB during anaphase
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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