| Gene name |
SPBC31F10.07 |
| Gene ID |
12/F12 |
| Gene synonyms/obsolete |
|
| Gene product |
actin cortical patch
component (putative); involved in actin cytoskeletal
organization; involved in endocytosis; couples endocytosis to
the cytoskeleton |
| Entry clone |
Cloned |
| ORF length (unspliced) |
915 |
| ORF length (spliced) |
|
| Entry clone length |
915 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC31F10.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAATTTTCAGTGAAAC |
| Rev primer name |
SPBC31F10.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCCAAACGATTATGATCG |
| Amino acid length |
304 |
| Molecular weight |
34.1 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; cytoplasmic dots
at periphery, especially at cell tip and site of septum
formation; nucleus>cytosol |
| Comments for localization |
asymmetrical
localization of SPB during anaphase |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |