Gene name |
SPAC23D3.07 |
Gene ID |
12/G03 |
Gene synonyms/obsolete |
|
Gene product |
20S proteasome
component (beta 2) |
Entry clone |
Cloned |
ORF length (unspliced) |
917 |
ORF length (spliced) |
804 |
Entry clone length |
917 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22D3.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGGGAATAAATGAAAG |
Rev primer name |
SPAC22D3.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACATCAACTTGCATTTCA |
Amino acid length |
267 |
Molecular weight |
29.3 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol;
nuclear envelope |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |