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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC24H6.01c
Gene ID 51/E04
Gene synonyms/obsolete SPAC23E2.04c; SPAPB21F2.01
Gene product hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1965
ORF length (spliced) 1767
Entry clone length 1965
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC24H6.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTACGTTTATTTCGATT
Rev primer name SPAC24H6.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTTACGCGAATTTGAAACA
Amino acid length 588
Molecular weight 69
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain 9
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFNITMLRL/LTYIFYAPLYL
Localization (YFP) cytosol?
Comments for localization too dark to photo
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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