Gene name |
SPBC29A10.14 |
Gene ID |
51/E03 |
Gene synonyms/obsolete |
rec8 |
Gene product |
involved in meiotic
recombination (required); cohesin complex (meiotic);
centromeric cohesin complex (meiotic); armassociated cohesin
complex (meiotic) |
Entry clone |
Cloned |
ORF length (unspliced) |
1873 |
ORF length (spliced) |
1686 |
Entry clone length |
1873 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC29A10.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTTACAATCAAGATGT |
Rev primer name |
SPBC29A10.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATGGCATCGGTGCTTTTT |
Amino acid length |
561 |
Molecular weight |
64 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
280 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LALRLSSNLMI |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
one nuclear
dot/nucleus |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |