| Gene name |
SPBC29A10.05 |
| Gene ID |
51/E02 |
| Gene synonyms/obsolete |
exo1 |
| Gene product |
exonuclease I; XP-G
family; involved in DNA repair; involved in mismatch repair;
5'-3' exonuclease; functions in the same pathway as msh2 and
pms1 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1716 |
| ORF length (spliced) |
|
| Entry clone length |
1716 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC29A10.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAATTAAAGGTCTTTT |
| Rev primer name |
SPBC29A10.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGAAATTTATATTGCTGA |
| Amino acid length |
571 |
| Molecular weight |
63.8 |
| Isoelectric point (calc.) |
9.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
89 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKYAIHQALML |
| Localization (YFP) |
nucleus; nuclear dots;
spindle microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |