Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC29A10.05
Gene ID 51/E02
Gene synonyms/obsolete exo1
Gene product exonuclease I; XP-G family; involved in DNA repair; involved in mismatch repair; 5'-3' exonuclease; functions in the same pathway as msh2 and pms1
Entry clone Cloned
ORF length (unspliced) 1716
ORF length (spliced)
Entry clone length 1716
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC29A10.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAATTAAAGGTCTTTT
Rev primer name SPBC29A10.05.Rv
Rev primer SEQ AGAAAGCTGGGTAACGAAATTTATATTGCTGA
Amino acid length 571
Molecular weight 63.8
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 89
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKYAIHQALML
Localization (YFP) nucleus; nuclear dots; spindle microtubules
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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