Gene name |
SPCC11E10.09c |
Gene ID |
51/E01 |
Gene synonyms/obsolete |
SPCC188.01c |
Gene product |
Sp specific families;
alpha-amylase; no apparent Sc orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
1546 |
ORF length (spliced) |
1437 |
Entry clone length |
1546 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC11E10.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCTAATCTGCTCCAT |
Rev primer name |
SPCC11E10.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAGGAAGCTAGGGTCAGAG |
Amino acid length |
478 |
Molecular weight |
55.4 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |