| Gene name |
SPAC12B10.14c |
| Gene ID |
49/D04 |
| Gene synonyms/obsolete |
|
| Gene product |
serine/threonine
protein kinase; similar to Sp pak1 and shk2 (paralogs) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2056 |
| ORF length (spliced) |
1998 |
| Entry clone length |
2056 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1741T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC12B10.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTCCAATTCTACATT |
| Rev primer name |
SPAC12B10.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAGATAACTGTAGCGAGAT |
| Amino acid length |
665 |
| Molecular weight |
74.5 |
| Isoelectric point (calc.) |
9.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery at cell tip;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |