Gene name |
SPAC9E9.12c |
Gene ID |
48/G02 |
Gene synonyms/obsolete |
abc1 |
Gene product |
Abc1 transporter; ABC
transporter family; MDR subfamily; similar to mammalian bile
acid transporter |
Entry clone |
Cloned |
ORF length (unspliced) |
4284 |
ORF length (spliced) |
|
Entry clone length |
4284 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
2841G:A |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC9E9.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAATGTTTCAGTCTTT |
Rev primer name |
SPAC9E9.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGACTAATATGACTTTCC |
Amino acid length |
1427 |
Molecular weight |
160.6 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
17 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFSLTNLFL/LLEYIQIILSI |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |