Gene name |
SPBC336.03 |
Gene ID |
47/F03 |
Gene synonyms/obsolete |
efc25 |
Gene product |
exchange factor
Cdc25p-like; guanine nucleotide exchange factor; GEF for
Ras1-Scd1 pathway; deletion can worsen the growth defect of
moe1; deletion synthetically lethal with tea1 deletion |
Entry clone |
Cloned |
ORF length (unspliced) |
2964 |
ORF length (spliced) |
|
Entry clone length |
2964 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC336.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAAGACCAAATTTGGA |
Rev primer name |
SPBC336.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGAAGAACGAGGTTCAAGT |
Amino acid length |
987 |
Molecular weight |
112.7 |
Isoelectric point (calc.) |
6.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNDRVINLFL/LSYLFDKSLSL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |