Gene name |
SPBC839.08c |
Gene ID |
47/F02 |
Gene synonyms/obsolete |
|
Gene product |
phosphatidylinositol
glycan, class N |
Entry clone |
Cloned |
ORF length (unspliced) |
2960 |
ORF length (spliced) |
2808 |
Entry clone length |
2960 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC839.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGGTCGCCTCTTGCT |
Rev primer name |
SPBC839.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTAACTTTCTCATGAATA |
Amino acid length |
935 |
Molecular weight |
106.4 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
14 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLKILFISLCI/LRLVAFGLFI/LDRLAILFL |
Localization (YFP) |
Golgi; vacuole
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |