Gene name |
SPBC17G9.04c |
Gene ID |
46/C11 |
Gene synonyms/obsolete |
|
Gene product |
similarity to
nucleoporin (nuclear pore complex) |
Entry clone |
Cloned |
ORF length (unspliced) |
2028 |
ORF length (spliced) |
|
Entry clone length |
2028 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC17G9.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATCTTCGGATGAAGT |
Rev primer name |
SPBC17G9.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTTTAAGTGAAGAAAA |
Amino acid length |
675 |
Molecular weight |
78 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDDFVVLLDI |
Localization (YFP) |
SPB?; nuclear dots;
nucleus>>cytosol; nuclear envelope |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |