| Gene name |
SPCC364.04c |
| Gene ID |
46/C10 |
| Gene synonyms/obsolete |
|
| Gene product |
CASP family protein; 1
predicted transmembrane helix |
| Entry clone |
Cloned
(Re-cloned) |
| ORF length (unspliced) |
2025 |
| ORF length (spliced) |
1902 |
| Entry clone length |
2025 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC364.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGTTGCTTCAGAAGC |
| Rev primer name |
SPCC364.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAATTCATAGGAGTATAT |
| Amino acid length |
633 |
| Molecular weight |
72.1 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGEMKGLLKL/LFIMIVLLKL |
| Localization (YFP) |
no transformant |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|