Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAP8A3.14c
Gene ID 46/C12
Gene synonyms/obsolete
Gene product hypothetical protein; involved in mitochondrial metabolism
Entry clone Cloned
ORF length (unspliced) 2034
ORF length (spliced)
Entry clone length 2034
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAP8A3.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAAAAGGATCATTATT
Rev primer name SPAP8A3.14.Rv
Rev primer SEQ AGAAAGCTGGGTAAGATATTAAACTTCTTTCA
Amino acid length 677
Molecular weight 77.9
Isoelectric point (calc.) 9.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.