Gene name |
SPBC19C7.10 |
Gene ID |
45/A08 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; APSES domain; DNA-binding protein; serine-lysine
rich protein; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1555 |
ORF length (spliced) |
1299 |
Entry clone length |
1555 |
No. of intron |
5 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC19C7.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGAGAATGAGAAAAG |
Rev primer name |
SPBC19C7.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAATAGAAAAGGTAAAATT |
Amino acid length |
432 |
Molecular weight |
47.8 |
Isoelectric point (calc.) |
9.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |