| Gene name |
SPBC14C8.15 |
| Gene ID |
45/A07 |
| Gene synonyms/obsolete |
|
| Gene product |
triglyceride
lipase-cholesterol esterase; similar to Sp SPCC1672.09 and
SPBC16A3.12C (paralogs) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1555 |
| ORF length (spliced) |
1383 |
| Entry clone length |
1555 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC14C8.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTCTGAATGGAAATAT |
| Rev primer name |
SPBC14C8.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATTGTATGGTTCATAACG |
| Amino acid length |
460 |
| Molecular weight |
52.6 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNYLPPVLFI |
| Localization (YFP) |
Golgi?; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |