Gene name |
SPAC23H4.10c |
Gene ID |
45/A09 |
Gene synonyms/obsolete |
thi4 |
Gene product |
bifunctional enzyme;
involved in thiamine biosynthesis; hydroxyethylthiazole
kinase; thiamine-phosphate dipyrophosphorylase |
Entry clone |
Cloned |
ORF length (unspliced) |
1557 |
ORF length (spliced) |
|
Entry clone length |
1557 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC23H4.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAACGTCAAATTGACTA |
Rev primer name |
SPAC23H4.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCAGCTTTCTCTAATTTG |
Amino acid length |
518 |
Molecular weight |
55.5 |
Isoelectric point (calc.) |
6.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol; cytoplasmic
dots |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |