Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC15C4.03
Gene ID 44/F06
Gene synonyms/obsolete
Gene product RAB geranylgeranyl-transferase escort protein; GDP dissociation inhibitor
Entry clone Cloned
ORF length (unspliced) 1429
ORF length (spliced) 1380
Entry clone length 1429
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC15C4.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGATCCAAATAGTTA
Rev primer name SPBC15C4.03.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATCATCCTCATCAAAT
Amino acid length 459
Molecular weight 52
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization occasionally a few cytoplasmic dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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