Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC365.02c
Gene ID 43/H07
Gene synonyms/obsolete cox10
Gene product involved in cytochrome c oxidase biogenesis; heme farnesyl transferase; involved in heme A synthesis
Entry clone Cloned
ORF length (unspliced) 1164
ORF length (spliced)
Entry clone length 1164
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC365.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTCATATACTAAACAA
Rev primer name SPBC365.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATATTTCAATGTATCCTCC
Amino acid length 387
Molecular weight 43.1
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain 6
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRYALAFLPL/LHLPLLFTLTL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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