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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC330.10
Gene ID 43/H08
Gene synonyms/obsolete pcm1
Gene product mRNA capping methyltransferase; methylates the terminal guanine residue of the mRNA cap
Entry clone Cloned
ORF length (unspliced) 1170
ORF length (spliced)
Entry clone length 1170
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC330.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATAGTCATCATATGAT
Rev primer name SPCC330.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAATTCCCCTCTTCTCAAAG
Amino acid length 389
Molecular weight 45
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LIWVKPFLDI
Localization (YFP) periphery at site of septum formation; cytosol=nucleus
Comments for localization cytoplasmic dots by over expression?
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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