Gene name |
SPCC330.10 |
Gene ID |
43/H08 |
Gene synonyms/obsolete |
pcm1 |
Gene product |
mRNA capping
methyltransferase; methylates the terminal guanine residue of
the mRNA cap |
Entry clone |
Cloned |
ORF length (unspliced) |
1170 |
ORF length (spliced) |
|
Entry clone length |
1170 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC330.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAGTCATCATATGAT |
Rev primer name |
SPCC330.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAATTCCCCTCTTCTCAAAG |
Amino acid length |
389 |
Molecular weight |
45 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIWVKPFLDI |
Localization (YFP) |
periphery at site of
septum formation; cytosol=nucleus |
Comments for localization |
cytoplasmic dots by
over expression? |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |