| Gene name |
SPCC1322.16 |
| Gene ID |
43/H06 |
| Gene synonyms/obsolete |
|
| Gene product |
prohibitin; involved
in cell aging; involved in proteolysis |
| Entry clone |
Cloned directly into
pDUAL-FFH1 |
| ORF length (unspliced) |
1152 |
| ORF length (spliced) |
840 |
| Entry clone length |
1152 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1322.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGATTTGATGAAGCG |
| Rev primer name |
SPCC1322.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTAATATCATCTAAAAGT |
| Amino acid length |
279 |
| Molecular weight |
31.1 |
| Isoelectric point (calc.) |
10.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|